Published September 2001
by Cold Spring Harbor Laboratory Press .
Written in English
|The Physical Object|
|Number of Pages||640|
Eukaryotic Transcriptional Regulation Like prokaryotic cells, the transcription of genes in eukaryotes requires the actions of an RNA polymerase to bind to a sequence upstream of a gene to initiate transcription. However, unlike prokaryotic cells, the eukaryotic RNA polymerase requires other proteins, or transcription factors, to facilitate Author: Lisa Bartee, Walter Shriner, Catherine Creech. Figure In Drosophila melanogaster, the sex is determined by a series of splicing events in sex determination genes on a cell-by-cell basis without any involvement of sex hormones (which circulate throughout the entire body).The primary sex-determination gene is Sex lethal (Sxl), which is transcribed only when the X/A ratio (the X chromosome-to-autosome ratio) equals or . The first edition of the highly successful Transcriptional Regulation in Eukaryotes, written by Michael Carey and Stephen Smale at UCLA, provided a comprehensive source of strategic, conceptual, and technical information for investigating the complexities of gene regulation at the level of transcription.5/5(1). Eukaryotic Translational and Post-Translational Regulation After the RNA has been transported to the cytoplasm, it is translated into protein. Control of this process is largely dependent on the RNA molecule. As previously discussed, the stability of the RNA will have a large impact on its translation into a : Lisa Bartee, Walter Shriner, Catherine Creech.
3 Transcriptional Regulation in Eukaryotes The familiar regularities that characterize coding sequences, in particular the genetic code, are absent from promoters. Understanding the functional consequences of evolutionary differences in promoter sequences therefore requires a clear knowledge of the mechanisms of transcriptional by: RNA is transcribed, but must be processed into a mature form before translation can begin. This processing that takes place after an RNA molecule has been transcribed, but before it is translated into a protein, is called post-transcriptional with the epigenetic and transcriptional stages of processing, this post-transcriptional step can also be regulated to . This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells. Chapters focus on the latest use of CRISPRi and RNAi technologies for studying various aspects of transcriptional and posttranscriptional regulation and. Use the following information for the next 3 problems. Let's imagine that part of the regulation of expression of the OB gene is mediated by a protein we will call OBF1. There is one binding site for OBF1 in the OB gene, and let's assume that is the only specific binding site in the haploid genome, or 2 specific sites in a diploid genome.
Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a 5/5(3). The evolution of transcriptional regulation in eukaryotes. Wray GA(1), Hahn MW, Abouheif E, Balhoff JP, Pizer M, Rockman MV, Romano LA. Collaborators: Wray GA(2). Author information: (1)Department of Biology, Duke University, USA. [email protected] (2)Duke U, Durham, NCCited by: Transcriptional Regulation in Eukaryotes. Carey, M., Smale, S. T.; Cold Spring Harbor Laboratory Press, , pp., ISBN 0‐‐‐4 (paperback), $ This book arose from the summer course in eukaryotic gene expression at Cold Spring Harbor Laboratory, in which the authors served as course instructors from through Author: Christopher K. Mathews. About this book Reflecting the rapid progress in the field, the book presents the current understanding of molecular mechanisms of post-transcriptional gene regulation thereby focusing on RNA processing mechanisms in eucaryotic cells.